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Deviant Agony 556 by kelbv Deviant Agony 556 by kelbv
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TheLastDanishPastry Featured By Owner May 30, 2009
Lysozyme (Muramidase) preferentially hydrolyses the Beta-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA (ref. 1 miniprep: Suppl. 15; pp. 1.6.4-7). The working concentration is 200 µg/300 µl. To increase the plasmid yield (approx. 5 - 10 %) in the so-called 'maxiprep', lysozyme may be added (ref. 1 maxiprep: Suppl. 41; pp. 1.7.2-4). Another application is the lysis of bacteria for the preparation of bacterial RNA (ref. 1 Suppl. 15; pp. 4.4.4-5). In this case the working concentration is 40 µg/ml (stock solution 50 mg/ml).
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May 30, 2009
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